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Description:P-values were determined using paired t-test. Chromatogram of a IMAC based affinity purification: Total protein lysate, lane 2: Only excellent grade oocytes with more than five compact layers of cumulus cells and homogeneous cytoplasm were used for in vitro maturation IVM and in vitro fertilization IVF. Sperm membrane integrity assessment The carboxyfluorescein-diacetate-propidium iodide CFDA-PI staining was performed according to the previously described method in buffaloes [ 33 ] with minor modifications. Systemic disorders such as polycystic kidney disease [ 4 ] also affect fertility and cause asthenozoospermia.

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